Invitrogen™ Mouse TPO (Thrombopoietin) (Carrier-Free) Recombinant Protein, eBioscience™

Recombinant Protein

Supplier:  Invitrogen™ 34868663

Catalog No. 50-112-3514

Description

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa. Description: Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa. Applications Reported: Recombinant human TPO is biologically active and can promote proliferation of human MO7e cells in culture. Applications Tested: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg. Source: E. coli (amino acids Glu22-Thr195; accession # NP_001166976.1). Bioactivity: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC. Molecular Weight: 18.7 kDa. Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water too a concentration of less than or equal to 0.5mg/mL. This protein dissolves slowly. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.

Specifications

• Accession Number: P40226
• Formulation: 0.01M tris with no preservative; pH 8.0
• Molecular Weight (g/mol): 18.7
• pH Range: 8.0
• Quantity: 10μg
• Storage Requirements: -20°C
• Structural Form: E. coli (amino acids Glu22-Thr195; Accession # NP_001166976.1)
• Common Name: Mouse Thrombopoietin (TPO) Carrier-Free
• Biological Activity: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 10⁶ Units/mg.
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant

• For Use With (Application): Control, Functional Assay
• Gene ID (Entrez): 21832
• Name: Mouse Thrombopoietin (TPO) Carrier-Free
• Purification Method: SDS-PAGE and HPLC
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.1ng/μg cytokine as determined by the LAL assay
• Gene Symbol: Thpo
• Product Type: Recombinant Protein, Carrier-Free
• Conjugate: Unconjugated
• Species: E. coli
• Form: Lyophilized
• Purity or Quality Grade: Affinity chromatography

Safety and Handling

ShelfLife : 12 Months