Learn More
MP Biomedicals™ Peroxidase From Horseradish
Useful for tracing neural connections, amplifying weak signals and increasing detectability of a target molecule such as a protein
Supplier: MP Biomedicals™ 0215003650
Description
A plant glycohemoprotein, Horseradish Peroxidase's (HRP's) enzymatic activity is due to the cyclic reduction and oxidation of the iron atom in the hematin group. The iron-containing hemin group is covalently bound to the glycoprotein apoenzyme. HRP readily combines with hydrogen peroxide and the resultant complex can oxidize a wide variety of chromogenic hydrogen donors. It is composed of seven isozymes, all containing photohemin IX as prosthetic group.
- Specific activity: ≥60 purpurogallin units/mg
- Extinction coefficient: EmM = 100 (403 nm)(Lit.)
- Presentation: brown powder
- Isoelectric Point: 7.2(Lit.)
- Optimum pH: 7.0(Lit.)
- Unit definition: amount of enzyme causing the production of 1mg of purpurogallin in 20 seconds at 20°C
RZ: ≥0.6 RZ Definition: The ratio of the absorbance at 403nm to the absorbance at 275nm. This value is an expression of the ratio of hemin to protein content.
Specifications
Brown | |
ELISA, immunoblotting, immunohistochemistry | |
Horseradish | |
Peroxidase | |
p-Aminobenzoic Acid, Azide, Cyclopropanone, L-cystine, Dichromate, Ethylenethiourea, Hydroxylamine, Sulfite and Vanadate and Divalent Metals Cd, Co, Cu, Fe, Mn, Ni, Pb |
7 | |
-20°C | |
50 kU | |
44000 | |
Lyophilized Powder |
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.