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MP Biomedicals™ Peroxidase From Horseradish

Useful for tracing neural connections, amplifying weak signals and increasing detectability of a target molecule such as a protein

Specifications

Color Brown
Product Type Peroxidase
Format Lyophilized Powder
For Use With (Application) ELISA, immunoblotting, immunohistochemistry
Inhibitors p-Aminobenzoic Acid, Azide, Cyclopropanone, L-cystine, Dichromate, Ethylenethiourea, Hydroxylamine, Sulfite and Vanadate and Divalent Metals Cd, Co, Cu, Fe, Mn, Ni, Pb
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
ICN15003650
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MP Biomedicals™
0215003650
50 kU This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A
Description

Description

A plant glycohemoprotein, Horseradish Peroxidase's (HRP's) enzymatic activity is due to the cyclic reduction and oxidation of the iron atom in the hematin group. The iron-containing hemin group is covalently bound to the glycoprotein apoenzyme. HRP readily combines with hydrogen peroxide and the resultant complex can oxidize a wide variety of chromogenic hydrogen donors. It is composed of seven isozymes, all containing photohemin IX as prosthetic group.

  • Specific activity: ≥60 purpurogallin units/mg
  • Extinction coefficient: EmM = 100 (403 nm)(Lit.)
  • Presentation: brown powder
  • Isoelectric Point: 7.2(Lit.)
  • Optimum pH: 7.0(Lit.)
  • Unit definition: amount of enzyme causing the production of 1mg of purpurogallin in 20 seconds at 20°C

RZ: ≥0.6 RZ Definition: The ratio of the absorbance at 403nm to the absorbance at 275nm. This value is an expression of the ratio of hemin to protein content.

Specifications

Specifications

Brown
Lyophilized Powder
p-Aminobenzoic Acid, Azide, Cyclopropanone, L-cystine, Dichromate, Ethylenethiourea, Hydroxylamine, Sulfite and Vanadate and Divalent Metals Cd, Co, Cu, Fe, Mn, Ni, Pb
7
Peroxidase
ELISA, immunoblotting, immunohistochemistry
44000
Horseradish
Safety and Handling

Safety and Handling

SDS
Product Certifications
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