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CD121a Rat anti-Mouse, Brilliant Violet 421, Clone: 35F5, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 564387

Catalog No. BDB564387

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The 35F5 monoclonal antibody specifically binds to the Type I Interleukin-1 Receptor (IL-1 RI) which is also known as CD121a. CD121a is expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80 kDa type-I transmembrane glycoprotein, a member of the Ig superfamily that mediates the inflammatory responses of leuc ocytes to IL-1. The 35F5 antibody is capable of blocking the binding of IL-1α, IL-1β, a nd IL-1 receptor antagonist to the Type I IL-1 Receptor. The 35F5 mAb is highly specific for the Type I IL-1 Receptor and does not react with the Type II IL-1 Receptor (CD121b) which is present on mouse macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.



Brilliant Violet 421
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
IgG1, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Il1r1; IL-1 Receptor Type I/p80; IL-1 R alpha; IL-1 Rα; IL-1 RI
Affinity Purified
IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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