CD140a Rat anti-Mouse, Brilliant Violet 510, Clone: APA5, BD Optibuild
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740148
The APA5 antibody monoclonal antibody specifically binds to CD140a, the Platelet-Derived Growth Factor Receptor alpha chain (PDGFR-a, PDGFR- α ). CD140a is a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult. CD140a is expressed on several other cell types during embryonic development, but not on hematopoietic cells. CD140a binds to PDGF A and B chains, in contrast to PDGFR-b (CD140b) that binds only to the PDGF B chain. Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization. The APA5 antibody has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo.
The antibody was conjugated to BD Horizon BV510 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.
|Brilliant Violet 510|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Pdgfra; Pdgfr2; PDGF-R alpha; Platelet derived growth factor receptor alpha|
|Mouse PDGF Receptor α chain|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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