CD29 Hamster anti-Mouse, Brilliant Violet 650, Clone: HM β1-1, BD Optibuild
Hamster Monoclonal Antibody
Manufacturer: BD Biosciences 740500
The HM β 1-1 monoclonal antibody specifically binds to the 130-kDa integrin β 1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin- α chains. With α 1 through α 6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with α v (CD51), it forms α α 8, and α 9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, epithelia, and oocytes. This hamster mAb to a mouse leukocyte antigen has been observed to crossreact with similar populations of rat leukocytes. Source of the immunogen was purified mouse VLA-4 ( α 4β 1, CD49d/CD29).
The antibody was conjugated to BD Horizon BV650 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Itgb1; Integrin beta-1; Fnrb; gpIIa; VLA-4 subunit beta|
|Purified mouse VLA-4|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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