MUC-1 Ab-5 Hamster Monoclonal Antibody, Epredia™

Epredia™ MUC-1 Ab-5, Hamster Monoclonal Antibody provides accurate, reproducible results.

Supplier:  Epredia HM1630R7

Catalog No. HM1630R7

Description

Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14-28kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind beta-catenin, GSK3beta and it associates with Grb2-SOS upon phosphorylation.

Host Species: Hamster

Clone: MH1 (CT2)

Isotype: IgG

Species Reactivity: Human and Mouse. Others not tested.

Epitope: aa 239-255

Immunogen: A synthetic peptide corresponding to aa 239-255 (SSLSYTNPAVAATSANL) from the cytoplasmic tail of MUC-1

Molecular Weight: 14-28kDa

Positive Control: MCF-7 cells; Breast CA

Cellular Localization: Cytoplasmic and membrane

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting
• Immunofluorescence
• Immunoprecipitation
• Flow Cytometry

Note:

USA: RUO; Int'l: RUO

Specifications

• Antigen: MUC-1 Ab-5
• Classification: Monoclonal
• Conjugate: Unconjugated
• Immunogen: A synthetic peptide corresponding to aa 239-255 (SSLSYTNPAVAATSANL) form the cytoplasmic tail of MUC1
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Prediluted

• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: MH1; same as CT2
• Host Species: Hamster
• Quantity: 7 mL
• Research Discipline: Cancer and Tumor Biology
• Target Species: Human, Mouse
• Isotype: IgG