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MUC-1 Mouse anti-Human, Clone: SPM132, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2328770.1MG

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Catalog No. NBP232877A


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Description

Description

MUC-1 Monoclonal antibody specifically detects Antigen in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting.
Specifications

Specifications

MUC-1
SPM132
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
P15941
Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, CD227, CD227 antigen, DF3 antigen, EMA, episialin, H23 antigen, H23AG, KL-6, MAM6, MUC-1, MUC1/ZD, mucin 1, cell surface associated, mucin 1, transmembrane, mucin-1, Peanut-reactive urinary mucin, PEMMUC-1/SEC, PEMT, Polymorphic epithelial mucin, PUMMUC-1/X, tumor associated epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
Mouse
IgG1 Kappa
0.1 mg
Cancer, Cellular Markers, Extracellular Matrix
Primary
4582
Human
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Unconjugated
PBS with 0.05% BSA. with 0.05% Sodium Azide
Purified
MUC1
Human milk fat globule membranes
Protein A purified
RUO
Store at 4C.
Monoclonal
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
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