The Human MUCIN 16/CA125 ELISA quantitates Hu Mucin 16 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Mucinn 16. Principle of the method The Human Mucin 16 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.CA125 determinant is present on a mucin-like glycoprotein of high molecular weight. CA125 has been found on frozen sections of amnion and derivatives of coelomic and mullerian epithelium, including pleura, pericardium and peritoneum. In adult tissues, epithelial cells of fallopian tube, endometrium and endocervix, pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland and mammary gland.
|2°C to 8°C|
|Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL|
|1 hr. 20 min.|
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