Description: This 14H1 monoclonal antibody recognizes human Musashi-1 (MSI-1), a 39 kDa RNA binding protein. Musashi-1 is expressed in CNS stem and progenitor cells with decreased expression in differentiating cells. Musashi-1 functions as a translational repressor, directly regulating its target proteins, Numb and p21 (CIP1). Musashi-1 has been shown to be expressed in stem cells in a variety of other tissues such as the gut, stomach, mammary gland, and hair follicles. In addition to normal tissue expression, Musashi-1 expression has been identified in cells from several tumor types, mainly pulmonary and adenocarcinoma, as well as large and small cell carcinomas. Recently, expression of Musashi-1 in ischemic lesions following stroke provides evidence for neurogenesis as well as the role of Musashi-1 in regulating apoptosis. Musashi-1 is highly conserved and the 14H1 antibody also recognizes mouse and rat Musashi-1. Applications Reported: This 14H1 antibody has been reported for use in immunoblotting (WB), immunohistology staining of paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This 14H1 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Musashi-1 belongs to the Musashi family of RNA-binding proteins which play a role in cell fate determination, and maintece of stem-cell state. Musashi family proteins are also involved in post transcriptional gene regulation. The gene encodes a protein with two conserved tandem RNA recognition motifs with highly conserved RNP (ribonucleoprotein) motifs. In mammals, Musashi-1 is expressed in fetal kidney, brain, liver and lung, and in adult brain and pancreas. In humans, the gene is located on chromosome 12.
|Human, Mouse, Rat|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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