BioVision Inc Myeloperoxidase (MPO) Fluorometric Activity Assay Kit: High-Throughput Assay for Measuring MPO Activity in Serum, Tissues, Cells etc. Simple, Sensitive and Reliable. Detection Limit: 0.5 μU. 100 Assays
Manufacturer: BioVision Inc K745100
Myeloperoxidase (MPO) Fluorometric Activity Assay Kit: Myeloperoxidase (MPO) is a peroxidase enzyme (EC 220.127.116.11) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. In BioVision's MPO Assay Kit, MPO catalyzes the production of NaClO from H?O? and NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/Em = 485/525 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5 ?U per well.
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