MYOD Monoclonal Antibody (5.8A), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA141017

Catalog No. 01-676-436

Description

The epitope of this antibody was mapped to a region within AA 180-189 of mouse MyoD. Suggested positive control: Rh-30.

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Specifications

• Antigen: MYOD
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: P10085, P15172
• Gene Symbols: MYOD1
• Immunogen: Recombinant mouse MyoD.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: 5.8A
• Conjugate: Unconjugated
• Gene: MYOD1
• Gene Alias: AI503393; bHLHc1; Class C basic helix-loop-helix protein 1; CMD1; MYF3; Myf-3; Myoblast determination protein 1; myoblast determination protein 1 homolog; Myod; MYOD1; Myod-1; MYOD1 homolog; myogenic differentiation 1; myogenic factor 1; myogenic factor 3; PUM; unnamed protein product
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 17927, 4654
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov