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Myosin 4, eFluor® 660, Clone: MF20, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 50650382

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Catalog No. 50-112-4425


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Description

Description

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis. The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog. Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections. Applications Tested: This MF20 antibody has been tested immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.

Muscle contraction. Weiss A., J. Mol. Biol. 290:61-75(1999). Denis N.J., Proteomics 7:868-874(2007).
Specifications

Specifications

Myosin 4
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q5SX39, Q28641, Q9Y623, Q076A5, Q29RW1
MYH2B, MyHC-2B, MyHC-IIb
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Bovine, Canine, Chicken, Chimpanzee, Feline, Guinea Pig, Human, Mouse, Non-human primate, Rabbit, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
MF20
eFluor™ 660
MYH4
Liquid
MYH4
IgG2b, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
479502, 468396, 4622, 100009548, 100726456, 360543, 17884, 408020
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