NEFL Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA110014

Catalog No. 01-670-271

Description

For Western blot using alkaline phosphatase plus chromagen, a dilution of at least 1:1000 is recommended. For chemiluminescence, use at a dilution of 1:20,000. Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

Specifications

• Antigen: NEFL
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NEFL
• Gene Alias: 68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; Nefl; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Human, Mouse, Pig, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Western Blot
• Concentration: Conc. Not Determined
• Formulation: whole serum with 5mM sodium azide
• Gene Accession No.: P02547, P02548, P07196, P08551, P19527
• Gene Symbols: NEFL
• Immunogen: Recombinant full length human NF-L protein
• Regulatory Status: RUO
• Gene ID (Entrez): 100521224, 18039, 281348, 4747, 83613
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid