Invitrogen™ NENF Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA520577

Catalog No. PIPA520577

Description

Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE. A suggested positive control is human small intestine tissue lysate. PA5-20577 can be used with blocking peptide PEP-0697.

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.

Specifications

• Antigen: NENF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NENF
• Gene Alias: 1110060M21Rik; cell growth-inhibiting protein 47; cell immortalization-related protein 2; CIR2; NENF; nenf {ECO:0000250; Neudesin; neudesin neurotrophic factor; neuron derived neurotrophic factor; neuron-derived neurotrophic factor; Protein GIG47; SCIRP10; SCIRP10-related protein; secreted protein of unknown function; sp2; SP2 protein; Spinal cord injury related protein 10; spinal cord injury-related protein 10; SPUF; SPUF protein; UniProtKB:Q9CQ45}; wu:fa11a04; wu:fb73f07; zgc:112953; zgc:112953 protein; zgc:152703
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 289380, 29937, 66208
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q6IUR5, Q9CQ45, Q9UMX5
• Gene Symbols: NENF
• Immunogen: A 15 amino acid peptide from near the carboxy terminus of human NENF.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov