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Nestin Mouse anti-Human, Clone: 10C2, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$124.00 - $345.00

Specifications

Antigen Nestin
Clone 10C2
Target Species Human
Conjugate Unconjugated
Isotype IgG1
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-138-22 Non-distribution product as customer accommodation.
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Invitrogen
14984380
25 μg
Each for $124.00
Add to cart
 
50-138-23 Non-distribution product as customer accommodation.
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Invitrogen
14984382
100 μg
Each for $345.00
Add to cart
 
Description

Description

Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody. Applications Reported: This 10C2 antibody has been reported for use in immunocytochemistry, microscopy, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and flow cytometric analysis. Applications Tested: This 10C2 antibody has been tested by western blot on SDS-reduced and non-reduced lysates from U251 cells and can be used at less than or equal to 5 µg/mL. The 10C2 antibody has also been tested by immunocytochemistry on formaldehyde-fixed and permeabilized SKNSH cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predomit marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
Specifications

Specifications

Nestin
Human
IgG1
Monoclonal
Liquid
Antibody
4° C
10C2
Unconjugated
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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