NGF Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA118377

Catalog No. 01-670-629

Description

Reconstitute in 500 μL of sterile, μgtrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8 °C. After reconstitution keep aliquots at -20 °C to -80 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

Specifications

• Antigen: NGF
• Classification: Polyclonal
• Formulation: PBS with no preservative
• Gene Accession No.: P01138, P01139, P25427
• Gene Symbols: NGF
• Immunogen: Native mouse beta NGF purified from submaxillary salivary gland.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Avian, Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Frozen), Inhibition Assays, Neutralization, Western Blot
• Conjugate: Unconjugated
• Gene: NGF
• Gene Alias: B nerve growth factor; beta ngf; beta-nerve growth factor; BetaNGF; beta-NGF; bNGF; b-NGF; HSAN5; MGC161426; MGC161428; nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta; nerve growth factor, beta polypeptide; nerve growth factor, beta subunit; NGF; NGF b; NGF β; Ngfb; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 18049, 310738, 4803
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized