The Human Nidogen-1 (Hu NID1) ELISA quantitates Hu NID1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NID1. Principle of the method The Human NID1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulfated glycoprotein that is widely distributed in basement membranes. It is tightly associated with laminin and also binds to collagen IV. Entactin probably has a role in cell-extracellular matrix interactions.
|2°C to 8°C|
|Plasma, 2 μL; Serum, 2 μL; Supernatant, 100 μL|
|1 hr. 20 min.|
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