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NOTCH1 Rat anti-Mouse, PE, Clone: 22E5, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 12576582

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Catalog No. 50-245-504


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Description

Description

Description: This 22E5 monoclonal antibody reacts with the extracellular domain of mouse Notch1, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription. Notch 1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells. Lower Notch1 expression levels can be found on B cells and monocytes. Studies show that some subsets of T-cell acute lymphoblastic leukemia (T-ALL) arise due to Notch1 chromosomal translocation with TCRbeta, which results in the expression of constitutively active Notch1. This cell surface receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation. Applications Reported: This 22E5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 22E5 antibody has been tested by flow cytometric analysis of mouse thymocytes.

This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).
Specifications

Specifications

Notch1
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q01705
9930111A19Rik; Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila); lin-12; major type A protein; Mis6; Motch A; mT14; N1; neurogenic locus notch homolog protein 1; Notch gene homolog 1; Notch homolog 1, translocation-associated; p300; RP23-306D20.12-001; Tan1; translocation-associated notch protein TAN-1; transmembrane receptor Notch1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
22E5
PE
Notch1
Liquid
Notch1
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
18128
SDS
Documents
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