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Notch2 Mouse anti-Human, Brilliant Violet 786, Clone: MHN2-25, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 742294

Catalog No. BDB742294


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Description

Description

The MHN2-25 monoclonal antibody specifically binds to an extracellular domain of human Notch2. Notch2 is a type 1 transmembrane glycoprotein receptor and member of the Notch family that includes Notch1-Notch4. Notch2 is cleaved in the Golgi and presents as a cell surface heterodimeric receptor. The Notch2 receptor can bind to several membrane-bound ligands including Jagged1, Jagged2, Delta1 and Delta4. Upon ligand binding, Notch2 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it forms a transcriptional activator complex with various transcriptional factors. These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of multiple organ systems such as the hematopoietic, immune, cardiovascular, hepatic and renal systems. Notch2 is expressed by cells of the lung and brain, as well as cells of the B lineage, thymocytes, and T cells. Abnormalities in Notch2 expression may play a role in the development of B cell lymphomas.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.
Specifications

Specifications

Notch2
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
NOTC2, AGS2, hN2, HJCYS, Neurogenic locus notch homolog protein 2
Human Notch2 Recombinant Protein
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Human
MHN2-25
Brilliant Violet 786
Affinity Purified
Murine
Mouse BALB/c IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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