NOTUM Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA112858

Catalog No. 01-675-838

Description

A suggested positive control for this product is HepG2 cell lysate.

Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the a/beta-hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.

Specifications

• Antigen: NOTUM
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOTUM
• Gene Alias: [Wnt protein] O-palmitoleoyl-L-serine hydrolase; 5730593N15Rik; hNOTUM; NOTUM; notum pectinacetylesterase homolog; notum pectinacetylesterase homolog (Drosophila); NOTUM, palmitoleoyl-protein carboxylesterase; OK/SW-CL.30; Palmitoleoyl-protein carboxylesterase NOTUM; protein notum homolog; RGD1307119
• Host Species: Rabbit
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 147111, 77583
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.08% sodium azide
• Gene Accession No.: Q6P988, Q8R116
• Gene Symbols: NOTUM
• Immunogen: Recombinant full-length notum protein from mouse.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG