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MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium-15N

Provides reagents to make a chemically defined autoinducing liquid culture medium used to produce [U-15N] labeled proteins for NMR spectroscopy. Novagen™ Overnight Express™ Autoinduction NMR Medium- 15N system includes four concentrated sterile solutions.

Manufacturer:  MilliporeSigma™ 717594

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Catalog No. 71-759-4


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Description

Description

  • Autoinduction method is based on the differential metabolism of carbon sources to promote growth to high cell density
  • Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG)
  • Includes four concentrated sterile solutions:
  • OnEx NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction
  • OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
  • OnEx Solution 3 provides magnesium for maximal cell density
  • OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels
Specifications

Specifications

Multiple storage requirements. Ok to freeze.
NMR Medium-15N
5 L Kit
SDS
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