Novex™ AcTEV™ Protease Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), ideal for removing affinity tags from fusion proteins

Manufacturer: Invitrogen 12575023

 View more versions of this product

Catalog No. 12-575-023

  Check Availability
Add to Cart


    Includes AcTEV™ Protease is supplied with a vial of 20X TEV buffer [1M Tris-HCl (pH 8.0), 10mM EDTA] and a vial of 100mM DTT.
    Product Type Protease
    Storage Requirements Store at -20°C. Guaranteed stable for 6 months when properly stored.
    Quantity 10,000 units

    An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

    • Highly specific cleavage activity
    • Enhanced enzyme stability for prolonged protease activity
    • Activity over a broad temperature (4°C to 30°C) and pH (6.0 to 8.5) range
    • Six-histidine sequence to facilitate its removal from the digested protein sample
    • Greater than 85% single-band purity with no nonspecific protease contamination

    • Application
      • Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag
      • This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant protein
      Enzyme specifications
      • Purified from E. coli expressing the AcTEV™ Protease gene
    • Unit definition
      • One unit of AcTEV™ Protease cleaves 85% of a 3µg control substrate in 1 hr at 30°C
    • Unit reaction conditions
      • 50mM Tris-HCl (pH 8.0), 0.5mM EDTA, 1mM DTT, 3µg control substrate, and 1 unit enzyme in 30µL for 1 hr at 30°C AcTEV™ Protease is functionally tested for the absence of any nonspecific protease activity

      GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

  • Description & Specifications

Related Products

Product Content Feedback

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit