Invitrogen™ IL-12 p40 Human ELISA Kit
Sandwich ELISA Kit
Manufacturer: Invitrogen™ KAC1561
The Human Interleukin-12 p40(Hu IL-12 p40) ELISA quantitates Hu IL-12 p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-12 p40. Principle of the method The Human IL-12 p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Recognizes natural and recombinant human IL-12p40.
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Shipping Condition: Wet ice
|Pre-coated 96 well plate,Standard,Sample diluent,Incubation Buffer,HRP conjugated Detection Antibody,Conjugate Diluent,Wash Buffer,Chromogen,Stop Solution,Controls|
|2°C to 8°C|
|4 hr. 30 min.|
|Colorimetric Microplate Reader|
|Plasma, 100 μL|Serum, 100 μL|Supernatant, 100 μL|
|1 hr. 20 min.|
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For Research Use Only. Not for use in diagnostic procedures.