Invitrogen™ HGF Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ KAC2211

Catalog No. KAC2211

Includes: Pre-coated plate, standard, controls, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Human Hepatocyte Growth Factor(Hu HGF) ELISA quantitates Hu HGF in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HGF. Principle of the method The Human HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Hepatocyte growth factor (HGF) is a member of the plasminogen subfamily of S1 peptidases. The inactive polypeptide formed upon translation undergoes cleavage to produce a 69 kDa alpha-chain and 34 kDa beta-chain. A disulphide bond between the two chains makes active HGF. HGF is secreted by mesenchymal cells and stimulates mitogenesis, cell motility, and matrix invasion. It is involved in cellular growth and morphogenesis in a wide variety of tissue types. In humans, HGF is present on chromosome 7.

Order Info

Shipping Condition: Dry Ice

Specifications

• Accession Number: P14210
• Assay Sensitivity: <20 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: DFNB39,F-TCFB,HPTA,SF,HGF
• Interassay CV: 5.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: HGF
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 125-8,000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Colorimetric Detection
• Gene ID (Entrez): 3082
• Incubation Time: 5 hr.
• Intraassay CV: 4.8%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Target: Human
• Total Assay Time: 5 hr.

For Research Use Only. Not for use in diagnostic procedures.