Invitrogen™ IL-12 p40/p70 Human ELISA Kit
Sandwich ELISA Kit
Manufacturer: Invitrogen™ KHC0121
DescriptionInterleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). Following protein synthesis, an active heterodimer (p70) and a homoodimer of p40 are formed. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-γ) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-γ and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.
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|Plasma, 50 μL|Serum, 50 μL|Supernatant, 50 μL|
|1 hr. 20 min.|
|Colorimetric Microplate Reader|
|Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers|
|2°C to 8°C|
|Plasma, Serum, Supernatant|
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For Research Use Only. Not for use in diagnostic procedures.