Invitrogen™ IL-12 p40/p70 Human ELISA Kit  

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ KHC0121

Catalog No. KHC0121

Description

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). Following protein synthesis, an active heterodimer (p70) and a homoodimer of p40 are formed. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-γ) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-γ and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

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Specifications

• Accession Number: P29459,P29460
• Assay Sensitivity: <2 pg/mL
• For Use With (Application): Colorimetric
• Gene ID (Entrez): 3592, 3593
• Interassay CV: 3.6%
• Kit Contents: Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
• Precision: <2pg/mL
• Quantity: 96 Tests
• Sample Type: Plasma,Serum,Supernatant
• Storage Requirements: 2°C to 8°C
• Target Species: Human
• Total Assay Time: 3 hr.

• Assay Range: 7.8-500 pg/mL
• Conjugate: Biotin
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CLMF,CLMF2,IL-12B,IMD28,IMD29,NKSF,NKSF2,CLMF,IL-12A,NFSK,NKSF1,P35
• Intraassay CV: 3.2%
• Label or Dye: HRP
• Product Type: ELISA Kit
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL|Serum, 50 μL|Supernatant, 50 μL
• Target: Human
• Test Time: 1 hr. 20 min.

For Research Use Only. Not for use in diagnostic procedures.