Invitrogen™ NuPAGE™ Large Protein Blotting Kit

Specifically designed for large protein analysis on NuPAGE Novex Tris-Acetate Gels under denaturing conditions

Supplier:  Invitrogen™ LP0001

Catalog No. LP0001

Description

The HiMark™ Pre-Stained and Unstained High Molecular Weight Protein Standards offer a time-saving load-and-go format. The HiMark Pre-stained Standard includes one pink colored orientation band and eight blue bands ranging in apparent molecular weight of 31 - 460kDa in NuPAGE Tris-Acetate Gel/SDS buffer system. It's suitable for monitoring electrophoresis, western transfer, and approximating molecular weight of unknown big proteins. The HiMark Unstained Standard, along with the NuPAGE Tris-Acetate Gel/SDS buffer system, offers the highest accuracy for molecular weight estimation of large proteins. It consists of nine proteins, ranging in size from 40 to 500 kDa in NuPAGE Novex 3-8% and 7% Tris-Acetate Gel/SDS buffer systems. Visualize with Coomassie™ stain, silver, and fluorescent protein stains post-electrophoresis. Several NuPAGE Large Protein Starter Kits are available to provide you with all necessary reagents and optimized protocols for high-resolution large protein separation and analysis. Each kit includes two boxes of NuPAGE 3-8% Tris-Acetate Gels, Tris-Acetate SDS Buffer Kit, a staining reagent or blotting membranes, and a vial of the appropriate HiMark Standard.

• Gel Percentage: 3-8 %
• Gel Size: Mini (8 x 8 cm)
• Well Format: 1D Well

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

• Content And Storage: See product manual for kit contents and component storage conditions. Components of each kit are also available individually.
• Gel Percentage: 3 to 8%
• Gel Type: Tris-Acetate
• Sample Loading Volume: 25 μL
• Stain Type: Unstained
• Product Line: NuPAGE™
• Thickness: 1.0 mm

• For Use With (Equipment): XCell SureLock Mini-Cell
• Gel Size: Mini
• Product Type: Protein Blotting Kit
• Separation Range: 40 to 500 kDa
• Wells: 10-well
• Quantity: 20 gels
• Well Design: 1D Well

For Research Use Only. Not for use in diagnostic procedures.