Invitrogen™ JAK2 (Phospho) [pY1007/pY1008] Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ KHO5621

Catalog No. KHO5621

Includes: JAK2 Antibody-Coated 96-Well Plate; JAK2 [pYpY1007/1008] Standard; Standard Diluent Buffer; JAK2 [pYpY1007/1008] Detection Antibody; anti-rabbit IgG-HRP (100X); HRP Diluent; Wash Buffer Concentrate (25X); Stabilized Chromogen, TMB; Stop Solution; Plate Covers; Detailed protocol with validation tests

Description

Kit is designed to detect and quantify the level of JAK2 (Phospho) [pY1007/pY1008] in fresh or frozen human or mouse cell lysates. The assay recognizes both natural and recombinant JAK2 (Phospho) [pY1007/pY1008]. Principle of the method A monoclonal capture antibody specific for JAK2 (Phospho) [pY1007/pY1008]) has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Janus Activating Kinase 2 (JAK2) is a non-rececptor tyrosine kinase involved in various processes such as cell growth, development, differentiation, and histone modifications. In response to a varitety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex. Mutations affecting this gene can cause Budd-Chiari syndrome, Polycythemia vera, Thrombocythemia 3, myelofibrosis, or Acute myelogenous leukemia.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: O60674,Q62120
• Assay Sensitivity: <1.5 U/mL
• Conjugate: HRP
• Sample Type: Cell Lysates
• Target Species: Human, Mouse
• Gene ID (Entrez): 16452, 3717
• Incubation Time: 4 hr.
• Intraassay CV: 4.5%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,10 μL
• Target: Human, Mouse
• Total Assay Time: 4 hr.

• Assay Range: 1.6-100 U/mL
• Concentration: 1.6U/mL
• Product Type: ELISA Kit
• Substrate Type: Cytoplasmic Tyrosine Kinases
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: JTK10,THCYT3
• Interassay CV: 7.5%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: JAK2
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

For Research Use Only. Not for use in diagnostic procedures.