BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.
The imm-Link amine conjugation kit allows amine containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilized mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.
Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.
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