Avidin is a 66kDa tetrameric glycoprotein composed of four identical subunits, which has widespread applications due to its very high affinity to the vitamin biotin. Activated avidin is covalently attached in a controlled process at near-neutral pH. The use of a stable avidin reagent at a low molar ratio of avidin:antibody, combined with highly efficient labeling process, means that desalting/dialysis steps are not needed and that avidin labeling can be carried out even at microgram scale without losses. The amount of antibody used for labeling should be the same as the pack size of LL-Avidin. For any new antibody, optimization of the ratio of antibody to dye is often worthwhile. If your antibody needs to be concentrated, purified, or cleaned up, consider purchasing an antibody clean up kit.
3 x 0.01mg Reactions
Store at -20°C. Avoid freeze-thaw cycles.
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher reagent
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