Unlike standard AMCA labeling procedures, where a large molar excess of AMCA is employed, the Lightning-Link AMCA kit combines a low molar ratio of AMCA:antibody and high labeling efficiency to eliminate the need for purification steps. AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in-situ hybridisation. It has an excitation wavelength of 345-350nm and an emission wavelength of 440-460nm.
3 x 200μg Reactions
Store at -20°C. Avoid freeze-thaw cycles.
Glass vial(s) of Lightning-Link Rapid mix (1 or 3 vials, depending on pack size), 1 vial of Rapid Modifier reagent, 1 vial of Rapid Quencher reagent
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