NSE Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA588488

Catalog No. PIPA588488

Description

Immunogen sequence: VIKDKYGKDA TNVGDEGGFA PNILENSEAL ELVKEAIDKA GYTEKIVIGM DVAASEFYRD GKYDLDFKSP TDPSRYITGD QLGALYQDFV RDYPVVSIED PFDQDDWAAW SKFTANVGIQ IVGDDLTVTN PKRIERAVEE KACNCLLLKV NQIGSVTEAI QACKLAQENG WGVMVSHRSG ETEDTFIADL VVGLCTGQIK TGAPCRSERL AKYNQLMRIE EELGDEARFA GHNFRNPSVL; Positive Samples: HeLa, LO2, SH-SY5Y, U-251MG, Mouse skeletal muscle, Mouse liver, Mouse brain, Rat brain, Rat skeletal muscle; Cellular Location: Cell membrane, Cytoplasm

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

Specifications

• Antigen: NSE
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ENO2
• Gene Symbols: Eno2
• Immunogen: Recombit fusion protein containing a sequence corresponding to amino acids 195-434 of human ENO2
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunofluorescence, Western Blot
• Concentration: 0.85 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P07323, P09104, P17183
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13807, 2026, 24334
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid