OPTIZYME™ Csp6I (CviQI), Fisher BioReagents™
5'...G^T A C...3'
3'...C A T^G...5'
Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl)2 and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 100%
- Buffer 2: 50 - 100%
- Buffer 3: 0 - 20%
- Buffer 4: 50 - 100%
- Buffer 5: 0 - 20%
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- 5'...m5C GTAm5C G...3'
|25 to 50% Water, >50% Glycerin|
|>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I|
|Keep container tightly closed|
|10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol|
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