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OPTIZYME™ Klenow Fragment, Fisher BioReagents™

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Catalog No. BP81061

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.



OPTIZYME™ Klenow Fragment is the large fragment of DNA Pol I and exhibits both 5'->3' polymerase and 3'->5' exonuclease activity.

Applications and Features:

  • OPTIZYME Klenow Fragment blunts DNA ends by filling in 5'-overhangs
  • Random-primed DNA labeling
  • Labeling by filling in 5'-overhangs of double-stranded DNA
  • Random-primed DNA labeling
  • DNA sequencing by the Sanger Method
  • Second strand synthesis of cDNA
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Active in buffers for OPTIZYME restriction enzymes, reverse transcriptases and T4 DNA ligase
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl, biotin-, digoxigenin-, and fluorescently-labeled nucleotides)
  • Lacks the 5'->3' exonuclease activity of DNA Polymerase I

Supplied with:
10X OPTIZYME Klenow Fragment Buffer: 500mM Tris-HCl (pH 8.0 at 25°C), 50mM MgCl2, 10mM DTT
Source: E. Coli cells with a cloned fragment of the polA gene
Quality Control:

  • Endodeoxyribonuclease-free
  • Functionally tested for fill in of 5'-overhanging DNA termini and for random primed DNA labeling



Klenow Fragment
E. coli cells with a cloned fragment of the polA gene
OPTIZYME™ Klenow Fragment Buffer 10X : Tris HCl 500mM (8 pH at 25°C), MgCl2 50mM, DTT 10mM
Klenow fragment blunts DNA ends by filling in 5'-overhangs, random-primed DNA labeling, DNA sequencing by the sanger method, second strand synthesis of cDNA, site-specific mutagenesis of DNA with synthetic Oligonucleotides, lacks the 5'->3' Exonuclease activity of DNA Polymerase I
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