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OPTIZYME™ SpeI (BcuI), Fisher BioReagents™

Catalog No. BP8018-1


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Description

Description

5'...A^C T A G T...3'
3'...T G A T C^A...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 0 - 20%
  • Buffer 4: 100%
  • Buffer 5: 20 - 50%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 80% of the DNA fragments can be ligated and more than 90% of these can be re-cut after 50-fold over-digestion with SpeI.
  • Digestion of Agarose-embedded DNA:
    • A minimum of 10 units of SpeI is required for the complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hours.
  • Note: Assayed using pUC19 DNA with insert, containing BcuI recognition site.
  • Compatible Ends: XmaJI, Eco130I, NheI, XbaI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: May overlap - effect not determined
  • EcoBI: May overlap - effect not determined
  • Specifications

    Specifications

    SacI
    10U/μL
    10X OPTIZYME™ Ecl136II, SacI Buffer
    7.4
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with SacI
    Liquid
    A.CTAGT
    37°C
    400U
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    SDS
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    Product Certifications
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