Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

OPTIZYME™ SpeI (BcuI), Fisher BioReagents™

Catalog No. BP8018-1

Order in Bulk

Request bulk or custom formats

Add to cart



5'...A^C T A G T...3'
3'...T G A T C^A...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 0 - 20%
  • Buffer 4: 100%
  • Buffer 5: 20 - 50%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 80% of the DNA fragments can be ligated and more than 90% of these can be re-cut after 50-fold over-digestion with SpeI.
  • Digestion of Agarose-embedded DNA:
    • A minimum of 10 units of SpeI is required for the complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hours.
  • Note: Assayed using pUC19 DNA with insert, containing BcuI recognition site.
  • Compatible Ends: XmaJI, Eco130I, NheI, XbaI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: May overlap - effect not determined
  • EcoBI: May overlap - effect not determined
  • Specifications


    10X OPTIZYME™ Ecl136II, SacI Buffer
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with SacI
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    Product Certifications
    Provide Content Correction

    We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Cancel Submit