OPTIZYME™ SpeI (BcuI), Fisher BioReagents™
5'...A^C T A G T...3'
3'...T G A T C^A...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 50 - 100%
- Buffer 2: 50 - 100%
- Buffer 3: 0 - 20%
- Buffer 4: 100%
- Buffer 5: 20 - 50%
- 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- A minimum of 10 units of SpeI is required for the complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hours.
|10X OPTIZYME™ Ecl136II, SacI Buffer|
|>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with SacI|
|10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol|
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