OPTIZYME™ T3 RNA Pol is a DNA-dependent RNA polymerase that catalyzes the 5'->3' synthesis of RNA from DNA.
Applications and Features:
- OPTIZYME™ T3 RNA Polymerase synthesizes unlabeled and labeled RNA that can be used for: (1) hybridization, (2) in vitro RNA translation, (3) RNase protection assays, (4) genomic DNA sequencing, (5) RNA splicing, and (6) studies of RNA secondary structure and RNA-protein interactions
- Incorporation of modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
5X OPTIZYME Transcription Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl2, 50mM DTT, 50mM NaCl, 10mM spermidine
Source: E. Coli cells with a cloned T3 RNA Polymerase gene
- Endo-, exodeoxyribonuclease and ribonuclease-free
- Functionally tested in in vitro transcription reaction
|T3 RNA Polymerase|
|E. coli cells with a cloned T3 RNA Polymerase gene|
|OPTIZYME™ Transcription Buffer 5X : Tris HCl 200mM (7.9 pH at 25°C), MgCl2 30mM, DTT 50mM, NaCl 50mM, Spermidine 10mM|
|Hybridization, in-vitro RNA translation, RNase protection assay, genomic DNA sequencing, RNA splicing and studies of RNA secondary structure and RNA-Protein interaction, quality control|
Safety and Handling
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