OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™

Catalog No. BP80995

Description

OPTIZYME™ T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Applications and Features:

• OPTIZYME™ T4 DNA Ligase is used to clone restriction enzyme generated DNA fragments
• Fast sticky end ligation (10min at room temperature)
• Cloning PCR products
• Ligation of double-stranded oligonucleotide linkers or adaptors to DNA
• Site-directed mutagenesis
• Amplified fragment length polymorphism
• Ligase-mediated RNA detection
• Nick repair in duplex DNA, RNA or DNA/RNA hybrids
• Active in buffers for OPTIZYME restriction enzymes

Supplied with:

• 10X OPTIZYME T4 DNA Ligase Buffer : 400mM Tris-HCl, 100mM MgCl₂, 100mM DTT, 5mM ATP (pH7.8 at 25°C)
• 50% OPTIZYME PEG 4000 Solution: 50% (w/v) polyethylene glycol

Source: E. Coli cells with a cloned gene 30 of bacteriophage T4
Quality control:

• Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
• Functionally tested for ligation of cohesive and blunt end DNA fragments

Specifications

• Concentration: 5 U/μL
• pH: 7.5
• Storage Buffer: OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)
• Source: E. coli cells with a cloned gene 30 of bacteriophage T4
• Product Type: T4 DNA Ligase

• Color: Colorless
• For Use With (Application): Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
• Content And Storage: -20°C
• Quantity: 500 U
• Form: Liquid