Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™

$71.00 - $145.00

Specifications

Color Colorless
Concentration 5U/μL
Product Type T4 DNA Ligase
Format Liquid
For Use With (Application) Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
View More Specs

Products 3
Catalog Number Quantity Price Quantity & Availability  
Catalog Number Quantity Price Quantity & Availability  
BP80991
View Documents
100U
Each for $71.00
Order in Bulk

Request bulk or custom formats

Add to cart
 
BP80995
View Documents
500U
Each for $145.00
Order in Bulk

Request bulk or custom formats

Add to cart
 
BP809915
View Documents
1500U This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

OPTIZYME™ T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Applications and Features:

  • OPTIZYME™ T4 DNA Ligase is used to clone restriction enzyme generated DNA fragments
  • Fast sticky end ligation (10min at room temperature)
  • Cloning PCR products
  • Ligation of double-stranded oligonucleotide linkers or adaptors to DNA
  • Site-directed mutagenesis
  • Amplified fragment length polymorphism
  • Ligase-mediated RNA detection
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids
  • Active in buffers for OPTIZYME restriction enzymes

Supplied with:

  • 10X OPTIZYME T4 DNA Ligase Buffer : 400mM Tris-HCl, 100mM MgCl2, 100mM DTT, 5mM ATP (pH7.8 at 25°C)
  • 50% OPTIZYME PEG 4000 Solution: 50% (w/v) polyethylene glycol
Source: E. Coli cells with a cloned gene 30 of bacteriophage T4
Quality control:
  • Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
  • Functionally tested for ligation of cohesive and blunt end DNA fragments

Specifications

Specifications

Colorless
T4 DNA Ligase
Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
E. coli cells with a cloned gene 30 of bacteriophage T4
-20°C
5U/μL
Liquid
7.5
OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)
SDS
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit