OPTIZYME™ T7 RNA Polymerase, Fisher BioReagents™

Catalog No. BP81021

Description

OPTIZYME™ T7 RNA Pol is a DNA-dependent RNA polymerase that catalyzes the 5'->3' synthesis of RNA from DNA.

Applications and Features:

• OPTIZYME T7 RNA Polymerase synthesizes unlabeled and labeled RNA that can be used for: (1) hybridization, (2) in vitro RNA translation, (3) RNase protection assays, (4) genomic DNA sequencing, (5) RNA splicing, and (6) studies of RNA secondary structure and RNA-protein interactions
• Incorporation of modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Supplied with:
5X OPTIZYME Transcription Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl₂, 50mM DTT, 50mM NaCl, 10mM spermidine
Source: E. Coli cells with a cloned T7 RNA Polymerase gene
Quality Control:

• Endo-, exodeoxyribonuclease and ribonuclease-free
• Functionally tested in in vitro transcription reaction

Specifications

• Concentration: 20 U/μL
• pH: 7.5
• Storage Buffer: OPTIZYME™ Transcription Buffer 5X : Tris HCl 200mM (7.9pH at 25°C), MgCl2 30mM, DTT 50mM, NaCl 50mM, Spermidine 10mM
• Quantity: 5000 U
• Form: Liquid

• Color: Colorless
• For Use With (Application): Hybridization, in-vitro RNA translation, RNase protection assay, genomic DNA sequencing, RNA splicing and studies of RNA secondary structure and RNA-Protein interaction, quality control
• Source: E. coli cells with a cloned T7 RNA Polymerase gene
• Product Type: T7 RNA Polymerase