OPTIZYME™ Terminal Deoxynucleotidyl Transferase, Fisher BioReagents™

Catalog No. BP81035

Description

OPTIZYME™ TdT is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH group of DNA

Applications and Features:

• OPTIZYME TdT produces synthetic homo- and heteropolymers
• Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus
• Oligodeoxyribonucleotide and DNA labeling
• 5'-Rapid Amplification of cDNA Ends (5'-RACE)
• In situ localization of apoptosis
• Incorporation of modified nucleotides (e.g., fluorescein-, biotin-, aminoallyl-labeled nucleotides)

Supplied with:
5X OPTIZYME TdT Buffer: 1M potassium cacodylate, 125mM Tris, 0.05% (v/v) Triton X-100, 5mM CoCl₂ (pH 7.2 at 25°C)

Source: E. Coli cells with a cloned gene encoding calf thymus terminal deoxynucleotidyl transferase

Quality Control:
Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free

Specifications

• Concentration: 20 U/μL
• pH: 7.5
• Storage Buffer: OPTIZYME™ TdT Buffer 5X : Potassium cacodylate 1M, Tris 125mM, Triton™ X-100 0.05%, CoCl2 5mM (7.2 pH at 25°C)
• Source: E. coli cells with a cloned gene encoding calf thymus terminal deoxynucleotidyl transferase
• Product Type: Terminal Deoxynucleotidyl Transferase

• Color: Colorless
• For Use With (Application): Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus, Oligodeoxyribonucleotide and DNA labeling, 5'-rapid amplification of cDNA Ends, in-situ localization of apoptosis incorporation of modified Nucleotides
• Content And Storage: -20°C
• Quantity: 1500 U
• Form: Liquid