Isolate Listeria spp. from food and environmental samples with Thermo Scientific Oxoid Demi-Fraser Broth (without Ferric Ammonium Citrate) (Dehydrated), when used as the secondary enrichment medium in methodology for Listeria isolation. This medium is a modification of Fraser Broth and contains half of the concentration of nalidixic acid and acriflavin hydrochloride to aid in the recovery of stressed cells. The removal of ferric ammonium citrate from the formula aids in subculture from broth to imaging instrumentation or Polymerase Chain Reaction-based assay machines.
Listeria monocytogenes is the most common pathogenic Listeria spp. and has been shown to be pathogenic to both humans and animals. Use Demi-Fraser Broth (without Ferric Ammonium Citrate) for isolation of Listeria species from food and environmental samples when used as the secondary enrichment medium in methodology for Listeria isolation.
- Helps in the recovery of stressed cells
- Allows subculture from the broth to imaging instrumentation or PCR based assay machines
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