Thermo Scientific™ P-PER™ Plant Protein Extraction Kit
Effectively extract protein from all kinds of dry and fresh plant tissue without liquid nitrogen using this plant cell-optimized kit.
Manufacturer: Thermo Scientific™ 89803
Effectively extract protein from all types of dry and fresh plant tissue without liquid nitrogen using Thermo Scientific™ P-PER Plant Protein Extraction Kit.The P-PER Kit includes an organic lysing reagent and two aqueous reagents that effectively lyse plant cells and solubilize protein without harsh mechanical lysis aids, such as a mortar and pestle. Plant protein extracts are prepared in just 10 minutes, and protein yields exceed conventional extraction methods (e.g., freeze/grinding in liquid nitrogen) and other commercially available plant protein extraction reagents. The resulting protein extracts are compatible with a variety of downstream applications including the Thermo Scientific BCA Protein Assay Kit - Reducing Agent Compatible (Part No. 23250), 1-D and 2-D gel electrophoresis, immunoprecipitation, Western blotting, activity assays and protein purification.
Versatile – works with multiple plant organs (leaf, stem, root, seed and flowers), multiple plant species (Arabidopsis, tobacco, maize, soybeans, peas, spinach and other plant tissues) and fresh, frozen and dehydrated plant tissues
Convenient – requires no liquid nitrogen/freeze-grinding, Dounce homogenization, blade-shearing or glass-bead agitation for cell disruption, although kit is compatible with these alternative mechanical aids
Compatible – downstream applications include 1-D and 2-D gel electrophoresis, Western blotting, activity assays, and protein affinity purifications
Quantifiable – kit extracts can be quantified using the BCA Protein Assay Kit, Reducing Agent Compatible
Ready to use – protein extract does not require filtration through cheesecloth or miracloth, unlike homebrews
Fast – perform plant cell lysis and protein extraction in 10 minutes
Recovers active protein – assays show extracted proteins are functional
P-PER Reagents A, B and C (40mL total); polypropylene bags
|P-PER Plant Protein Extraction Kit|
|Total of 400mg dried or 1.6g fresh plant tissue|
|P-PER Reagent A, 20mL, P-PER Reagent B, 225uL, P-PER Reagent C, 20mL, Polypropylene Mesh Bags, 20 ea|