Thermo Scientific™ P-PER™ Plant Protein Extraction Kit

Effectively extract protein from all kinds of dry and fresh plant tissue without liquid nitrogen using this plant cell-optimized kit.

Supplier:  Thermo Scientific™ 89803

Catalog No. PI89803

Description

Effectively extract protein from all types of dry and fresh plant tissue without liquid nitrogen using Thermo Scientific™ P-PER Plant Protein Extraction Kit.

The P-PER Kit includes an organic lysing reagent and two aqueous reagents that effectively lyse plant cells and solubilize protein without harsh mechanical lysis aids, such as a mortar and pestle. Plant protein extracts are prepared in just 10 minutes, and protein yields exceed conventional extraction methods (e.g., freeze/grinding in liquid nitrogen) and other commercially available plant protein extraction reagents. The resulting protein extracts are compatible with a variety of downstream applications including the Thermo Scientific BCA Protein Assay Kit - Reducing Agent Compatible (Part No. 23250), 1-D and 2-D gel electrophoresis, immunoprecipitation, Western blotting, activity assays and protein purification.

Highlights:

• Versatile – works with multiple plant organs (leaf, stem, root, seed and flowers), multiple plant species (Arabidopsis, tobacco, maize, soybeans, peas, spinach and other plant tissues) and fresh, frozen and dehydrated plant tissues

• Convenient – requires no liquid nitrogen/freeze-grinding, Dounce homogenization, blade-shearing or glass-bead agitation for cell disruption, although kit is compatible with these alternative mechanical aids

• Compatible – downstream applications include 1-D and 2-D gel electrophoresis, Western blotting, activity assays, and protein affinity purifications

• Quantifiable – kit extracts can be quantified using the BCA Protein Assay Kit, Reducing Agent Compatible

• Ready to use – protein extract does not require filtration through cheesecloth or miracloth, unlike homebrews

• Fast – perform plant cell lysis and protein extraction in 10 minutes

• Recovers active protein – assays show extracted proteins are functional

Includes:

P-PER Reagents A, B and C (40mL total); polypropylene bags

Specifications

• Description: P-PER Plant Protein Extraction Kit
• Quantity: 40mL kit
• Format: Liquid
• Source Material: Plant tissue

• Formulation: Four-component kit
• Sufficient For: Total of 400mg dried or 1.6g fresh plant tissue
• Kit Contents: P-PER Reagent A, 20mL, P-PER Reagent B, 225uL, P-PER Reagent C, 20mL, Polypropylene Mesh Bags, 20 ea