p21/CIP1/CDKN1A Antibody (CIP1/2275R), Novus Biologicals™

Rabbit Monoclonal Antibody

Supplier:  Novus Biologicals NBP280488

Catalog No. NB013559

Description

p21/CIP1/CDKN1A Monoclonal specifically detects p21/CIP1/CDKN1A in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: p21/CIP1/CDKN1A
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Western Blot 1 - 2 μg/mL, Immunohistochemistry, Immunohistochemistry-Paraffin
• Gene Accession No.: P38936
• Gene Symbols: CDKN1A
• Immunogen: Recombinant full-length human p21/CIP1/CDKN1A protein (Uniprot: P38936)
• Quantity: 20 μg
• Research Discipline: Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific
• Gene ID (Entrez): 1026
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Form: Purified

• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Clone: CIP1/2275R
• Conjugate: Unconjugated
• Formulation: 10 mM PBS with 0.05% BSA
• Gene Alias: CAP20cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDKN1melanoma differentiation associated protein 6, CIP1WAF1CDK-interaction protein 1, cyclin-dependent kinase inhibitor 1A (p21, Cip1), MDA6, MDA-6, Melanoma differentiation-associated protein 6, p21, p21CIP1, p21Cip1/Waf1, PIC1, SDI1DNA synthesis inhibitor, wild-type p53-activated fragment 1
• Host Species: Rabbit
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
• Content And Storage: Store at 4°C.
• Isotype: IgG