p27/Kip1 Mouse, Clone: DCS-72.F6 + KIP1/769, Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247771A	Novus Biologicals NBP2477710.1MG	0.1 mg
NBP247771B	Novus Biologicals NBP2477710.2MG	0.2 mg

Description

p27/Kip1 Monoclonal specifically detects p27/Kip1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: p27/Kip1
• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Prostate Cancer, Tumor Suppressors
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 1027
• Immunogen: Mouse recombinant p27 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 25.5 kDa

• Clone: DCS-72.F6 + KIP1/769
• Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Monkey
• Gene Alias: CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
• Gene Symbols: CDKN1B
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.