Novus Biologicals™ p53 Antibody Pair [HRP]

Mouse Monoclonal Antibody Pair

Supplier:  Novus Biologicals™ NBP27965815PLATES

Catalog No. NB010355

Description

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Cynomolgus Monkey p53.

Specifications

• Assay Range: 156.25 pg/mL
• Assay: Sandwich ELISA
• Immunoassay Kit Format: Sandwich ELISA Kit
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Cynomolgus Monkey p53.
• For Use With (Application): Sandwich ELISA
• Gene ID (Entrez): 7157
• Host Species: Mouse
• Quantity: 15 Plates
• Research Discipline: Apoptosis, Cancer, Cell Cycle and Replication, Cellular Markers, Checkpoint signaling, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, HIF Target Genes, Hypoxia, Neuroscience, Neurotransmission, p53 Pathway, Phospho Specific, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors
• Target: p53

• Assay Sensitivity: 156.25-10000 pg/mL
• Conjugate: HRP
• Product Type: Antibody Pairs
• Target Species: Cynomolgus Monkey
• Gene Alias: Antigen NY-CO-13, FLJ92943, LFS1TRP53, p53, p53 tumor suppressor, P53cellular tumor antigen p53, Phosphoprotein p53, transformation-related protein 53, tumor protein p53, Tumor suppressor p53
• Gene Symbol: TP53
• Kit Contents:
• Standard: Each vial contains 160 ng of recombinant p53.
• Capture Antibody: 1 mg/mL of mouse anti-p53 monoclonal antibody (in PBS, pH 7.4).
• Detection Antibody: 0.2 mg/mL of mouse anti-p53 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.