Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Supplier:  Thermo Scientific™ 26614

Catalog No. PI26614

Description

Thermo Scientific™ Unstained Protein Molecular Weight Marker is a mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Each protein in the ladder contains an integral Strep-tag™ II Sequence which can be detected directly on Western blots using a Strep-Tactin™ Conjugates or an antibody against Strep-tag™ II Sequence. The Ladder produces sharp bands on SDS-polyacrylamide gel following staining of the gel with Thermo Scientific Pierce Gel Stains or other coomassie dye or silver stains. The 50kDa protein band is of greater intensity and serves as a reference band.

Highlights:

• Size range – 14 proteins spanning 10 to 200kDa

• Ready-to-use – supplied in a loading buffer for direct loading on gels

• Sharp bands – excellent accuracy for detection in with coomassie or silver stains

• Reference band – 50kDa band is has greater intensity for easy orientation

• Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence

• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins (0.02 to 0.05mg/mL of each) in 62.5mM Tris-H³PO⁴ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN₃, 0.01% bromophenol blue and 33% glycerol.

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots

Specifications

• Content And Storage: Contents: two vials of 250 μL each, proteins at 0.02 to 0.05 mg/mL of each
  
  Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 0.01% bromophenol blue and 33% glycerol
  
  Storage: Upon receipt store at -20°C
  
  
• Product Type: Protein Ladder
• Size Range: 10 to 200 kDa
• Gel Compatibility: Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
• Product Line: PageRuler™
• Shipping Condition: Approved for shipment on Wet or Dry Ice

• Number of Markers: 14
• Ready to Load: Yes
• Stain Type: Unstained
• Molecular Weight (g/mol): 200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa
• Quantity: 2 x 250 mL
• System Type: Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.