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Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Manufacturer:  Thermo Scientific™ 26614

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Catalog No. PI26614


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Description

Description

Thermo Scientific™ Unstained Protein Molecular Weight Marker is a mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Each protein in the ladder contains an integral Strep-tag™ II Sequence which can be detected directly on Western blots using a Strep-Tactin™ Conjugates or an antibody against Strep-tag™ II Sequence. The Ladder produces sharp bands on SDS-polyacrylamide gel following staining of the gel with Thermo Scientific Pierce Gel Stains or other coomassie dye or silver stains. The 50kDa protein band is of greater intensity and serves as a reference band.

Highlights:

  • Size range – 14 proteins spanning 10 to 200kDa

  • Ready-to-use – supplied in a loading buffer for direct loading on gels

  • Sharp bands – excellent accuracy for detection in with coomassie or silver stains

  • Reference band – 50kDa band is has greater intensity for easy orientation

  • Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins (0.02 to 0.05mg/mL of each) in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol.

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots

Specifications

Specifications

Contents: two vials of 250 µL each, proteins at 0.02 to 0.05 mg/mL of each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Storage: Upon receipt store at -20°C

Protein Ladder
10 to 200 kDa
Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
PageRuler™
Approved for shipment on Wet or Dry Ice
14
Yes
Unstained
200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa
2 x 250 mL
Western Blotting, SDS-PAGE
SDS
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