Invitrogen™ PAK3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529075

Catalog No. PIPA529075

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, NIH-3T3, JC, mouse brain, rat brain. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (99%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

Specifications

• Antigen: PAK3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PAK3
• Gene Alias: 2.7.11.1; adriamycin resistance-associated; ARA; beta-PAK; bPAK; CDC42/RAC effector kinase PAK-B; CDKN1A; hPAK3; MRX30; MRX47; oligophrenin-3; OPHN3; p21 (CDKN1A)-activated kinase 3; p21 (RAC1) activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 3; p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p21-activated kinase 3; p65-PAK; PAK beta; Pak3; Pak-3; PAK3beta; Pak65alpha; Pak65beta; Pakb; Serine/threonine-protein kinase PAK 3; Stk4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18481, 29433, 5063
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.74 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O75914, Q61036, Q62829
• Gene Symbols: PAK3
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 253 and 544 of Human PAK3.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG